Stop Santino before he beats up the Courtesan.
You'll find another one in front of the bottom of the main staircase that leads to the Villa. Faction discount: You enter free running mode again and can run away. Like the Horseback foes, they have particular "cone-ish" like helmets.
Just follow Machiavelli for a while on horse. Poison fifteen guards. Ignore the other enemies and chase after him.
Then shimmy over to Desmond's left, automatically going across small gaps to the next part. Climb up the wall here to reach another beam. Blacksmiths sell weapons, armor and ammunition. Spartan War Hero Armor. Erom here, jump to Tiber Island via the wooden pole, then sprint to the target icon.
After the Assassins depart, the ability cannot be reused until the Assassin Signal is completely red. Art Connoisseur Bronze: Do an Air Assassination, or jump down and chase him before he gets away.
Of course, the easiest way might be to not worry about chaining kills together and just play a bit more defensively, using counter kills mostly. The wooden framework on the central pillar is used to reach each floor level. Ezio must stay within range of Micheletto as he walks through the Restricted Area, but avoid detection by other guards.
Speak to the highlighted individual to initiate each task. If you can make it, then good. Portraits Defeat the following targets to unlock the corresponding portrait: Interact to start a scene and the mission.